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Tris-buffered saline : ウィキペディア英語版 | Tris-buffered saline
Tris-buffered saline (abbreviated TBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. Tris (with HCl) has a slightly alkaline buffering capacity in the 7–9.2 range. Tris has a pKa of 8.06 at 25 °C. The pKa declines approximately 0.03 units per degree Celsius rise in temperature. This can lead to relatively dramatic pH shifts when there are shifts in solution temperature. ==Applications== TBS has many uses because it is isotonic and non-toxic. It can be used to dilute substances. Additives can be used to add function. TBS is often used in immuno-blotting for both membrane washing and antibody dilution.
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「Tris-buffered saline」の詳細全文を読む
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